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cpac imaging pro 5 free download full versionRUNX3 is a tumor suppressor gene that is silenced in a number of epithelial cancers including breast, prostate, and lung. In addition, RUNX3 expression is down-regulated in colon cancer. RUNX3 is a transcription factor that has been shown to inhibit cell growth and induce differentiation in several experimental systems. RUNX3 has been reported to have tumor suppressive function in prostate cancer cell lines and sporadic prostate tumors in humans. It is not clear why RUNX3 is inactivated in different types of epithelial cancers. Here we show that RUNX3 is repressed by hypermethylation in prostate cancer cell lines. We found a strong inverse correlation between RUNX3 expression and hypermethylation in prostate cancer cell lines. In addition, RUNX3 expression was restored in prostate cancer cells following treatment with a demethylating agent. Our data suggest that RUNX3 methylation is a mechanism of inactivation in prostate cancer cell lines. In addition, we found that a silent RUNX3 allele is present in breast cancer cell lines and breast tumors. We also demonstrate that RUNX3 is down-regulated in breast tumors. Therefore, our data suggest that inactivation of RUNX3 is a mechanism of tumorigenesis in both prostate and breast cancer. We are currently examining the methylation status of RUNX3 in human breast cancer tumors and normal breast tissue from breast cancer patients to determine if RUNX3 methylation is a mechanism of tumorigenesis in breast cancer as well.
The rate of birth of the hematopoietic system and gastrointestinal system is controlled in development of embryonal stem cells by specific extracellular stimuli. The signaling pathways involved in the fate decision of these two lineages are not well known. Here we examined the expression of transcripts in embryonic stem cells (ES cells) undergoing differentiation into these line be359ba680
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